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Title   위암세포주에서 p53 유전자 형질도입이 위암세포주의 Malignant Phenotype 에 미치는 영향 ( Effect on Malignant Phenotype of Gastric Cancer Cell Line after p53 Gene Transduction )
Publicationinfo   1998 Jan; 030(03): 508-521.
Key_word   Wild-type p53, Malignant phenotypes, Transduction, Gastric cancer
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Abstract   Purpose: To evaluate the effect of wild type p53 gene transduction on the malignant phenotypes for metastasis in gastric cancer, we compared the biological phenpotypes of gastric cancer cell lines based on p53 gene status. Then, after retrovirus-mediated wild-type p53 gene transduction, we compared those phenotypes among parent YCC-3 cell line, vector transduced YCC-3v cell line and a clone of YCC-3Cⓒy. Material and methods: Four human gastric cancer celi lines were used; YCC-l(mutant), YCC-2(wild), YCC-3(mutant) and AGS(wild). DNAs of the cell lines were analyzed to evaluate the mobility shift with PCR-SSCP. Tumorigenecity and proliferation were evaluated by soft agar assay and proliferation assay. Migratory capacity was measured by adhesion assay and Boyden chamber assay. p53 protein expression was measured by Western blot analysis and VEGF, WAF-1 were measured by ELISA assay. Angiogenic activity was measured by cross-feeding assay and cell cycle analysis was performed by flowcytometry. In vivo tumorigenicity was measured by xenograft in nude mice. Results: YCC-3 cell line with mutant p53 gene expressed all the phenotypes for the metastasis such as tumorigenicity, migration and angiogenesis. In a stable clone of YCC-3Cⓒy, no differences were found in proliferation, cell cycle and WAP-1 expression when compared to those of the control YCC-3v and parent YCC-3 cell line, even if increased p53 protein production was found by Western blot analysis. However, both in vitro and in vivo tumorigenicity were decreased in a stably transduced YCC-3Cⓒy clone. The adhesive capacity was also decreased in YCC-3Cⓒy clone whereas the endothelial cell growth stimulatory effect and VEGF production showed no difference compared to those of the YCC-3v cell line. Conclusion: Wild-type p53 gene transduction in gastric cancer cell line decreased tumorigenicity which resulted from decreased colony forming activity and adhesive capacity but not formed changes of angiogenic activity. This suggested the possible application of anti- metastasis strategy with p53 gene therapy in gastric cancer.
저 자   라선영(Sun Young Rha),김태수(Tae Soo Kim),정숙정(Sook Jung Jeong),안중배(Joong Bae Ahn),심광용(kwang Yong Shim),공수정(Soo Jung Kong),이화영(Hwa Young Lee),유내춘(Nae Choon Yoo),최진혁(Jin Hyuk Choi),임호영(Ho Young Lim),김주항(Joo Young Lim),노재경(Jae Kyung Roh),민진식(Jin Sik Min),김병수(Byung